primary antibody against muc6  (Boster Bio)

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: GO term enrichment analysis of DEGs 1 h after 10 mGy radiation exposure.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: Common upregulated genes.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: LDR IR increases G6PC2 and MUC6 mRNA and protein expression immediately exposure. (A) Schematic diagram of the experimental procedure. In experiment 1, mice irradiated with sham (0 Gy) or 10 mGy were sacrificed for tissue sampling at 1 or 24 h following LDR IR. Red square indicates radiation exposure time; black circles indicate the times of tissue collection from test animals. Functional enrichment analysis of DEGs. (B) Changes in mRNA expression of four selected genes in the jejunum of mice with LDR IR. Protein expression validation by western blot analysis and temporal changes in protein levels of upregulated genes in 10 mGy-irradiated jejunum. (C) Representative western blotting of ATP1A4, G6PC2, MUC6 and TRPV6 in the jejunum. (D) Bar graphs; expression was normalized to β-actin. Data are expressed as the mean ± SEM (n=3 mice per group). *P<0.05 and **P<0.01 vs. the sham group. LDR IR, low-dose-rate irradiation; G6PC2, glucose-6-phosphatase catalytic subunit 2; MUC6, mucin 6; DEGs, differentially expressed genes; ATP1A4, Na + /K + transporting subunit alpha 4; TRPV6, transient receptor potential cation channel subfamily V member 6.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques: Expressing, Irradiation, Sampling, Functional Assay, Western Blot

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: MUC6 mRNA increases in low dose of LDR IR and repeated LDR IR exposure conditions in the jejunum. (A) Schematic diagram of the experimental procedure. In experiment 2, mice exposed to LDR IR with 0, 10, 100, or 500 mGy or HDR IR with 500 mGy, then sacrificed at 1 or 24 h following IR. Red square indicates radiation exposure time; black circles indicate the times of tissue collection from test animals. Changes in the mRNA expression of MUC6 in the intestine of mice exposed to LDR IR. Dose-related changes in MUC6 mRNA expression level in (B) jejunum and (C) colon. (D) Schematic diagram of the experimental procedure. In experiment 3, mice irradiated with sham, 10 mGy once, or 10 mGy three times were sacrificed for tissue sampling at 1 or 24 h following LDR IR (n=3 mice per group). Red square indicates radiation exposure time; black circles indicate the times of tissue collection from test animals. (E) Repetition-related changes in MUC6 mRNA expression level in jejunum. Data are expressed as the mean ± SEM (n=3 mice per group). **P<0.01 and ***P<0.001 vs. the sham group. MUC6, mucin 6; LDR IR, low-dose-rate irradiation; HDR IR, high-dose-rate irradiation.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques: Expressing, Irradiation, Sampling

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: DSS and LDR IR did not alter jejunal morphology but increased G6PC2 and MUC6 mRNA expression. (A) Schematic diagram of the experimental procedure. In experiment 4, DSS-induced IBD model mice irradiated with 10 mGy three times were sacrificed for tissue sampling at 1 or 24 h following LDR IR. Red square indicates radiation exposure time; gray square indicates DSS treatment period; black circles indicate the times of tissue collection from test animals. (B) Representative images of hematoxylin and eosin-stained jejunal sections harvested from vehicle- or DSS-treated mice at 24 h after 10 mGy 3 times LDR IR. Changes in the mRNA expression of (C) G6PC2 and (D) MUC6 in the jejunum of mice exposed to DSS and LDR IR. Data are expressed as the mean ± SEM (n=3 mice per group). *P<0.05, **P<0.01 and ***P<0.001 vs. the sham group; ## P<0.01 and ### P<0.001 vs. the DSS group; ++ P<0.01 and +++ P<0.001 vs. the 10 mGy X3 times group. DSS, dextran sodium sulfate; LDR IR, low-dose-rate irradiation; G6PC2, glucose-6-phosphatase catalytic subunit 2; MUC6, mucin 6; IBD, inflammatory bowel disease.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques: Expressing, Irradiation, Sampling, Staining

Journal: Diagnostic Pathology

Article Title: Mucins Differently Expressed in Various Ampullary Adenocarcinomas

doi: 10.1186/1746-1596-6-102

Figure Lengend Snippet: MUC gene expression and logistic regression analysis of associations between MUC family genes and clinical characteristics

Article Snippet: The solution of 10% goat serum and primary antibody against MUC6 were obtained from Boster Biotech (Wuhan, Hubei, China).

Techniques: Gene Expression

Journal: Diagnostic Pathology

Article Title: Mucins Differently Expressed in Various Ampullary Adenocarcinomas

doi: 10.1186/1746-1596-6-102

Figure Lengend Snippet: Survival curves of the expression of MUC family genes . Kaplan-Meier curves analyze the relationships between survival and expression of MUC1 (A), MUC2 (B), MUC5AC (C), and MUC6 (D), where those patients with tumors negative for the gene expression are shown with solid lines, and those with tumors positive for the gene expression are shown with dashed lines. The open circles (○) indicate censored patients with tumors negative for the gene expression, and the open squares (□) indicate censored patients with tumors positive for the gene expression.

Article Snippet: The solution of 10% goat serum and primary antibody against MUC6 were obtained from Boster Biotech (Wuhan, Hubei, China).

Techniques: Expressing, Gene Expression

Journal: Diagnostic Pathology

Article Title: Mucins Differently Expressed in Various Ampullary Adenocarcinomas

doi: 10.1186/1746-1596-6-102

Figure Lengend Snippet: Survival curves of the expression of MUC family genes by different tumor location . Kaplan Meier curves analyze the relationships between survival and location of tumors for MUC1 (A), MUC2 (B), MUC5AC (C), and MUC6 (D), for tumors located in (1) the lower end of the common bile duct, (2) the papillary duodenum, and (3) the ampullary duodenum. The solid lines indicate tumors that were negative for the MUC gene expression, the dashed lines indicate tumors that were positive for the MUC gene expression, the open circles (○) indicate censored patients with tumors negative for the gene expression, and the open square (□) indicate censored patients with tumors positive for the gene expression.

Article Snippet: The solution of 10% goat serum and primary antibody against MUC6 were obtained from Boster Biotech (Wuhan, Hubei, China).

Techniques: Expressing, Gene Expression

Journal: Diagnostic Pathology

Article Title: Mucins Differently Expressed in Various Ampullary Adenocarcinomas

doi: 10.1186/1746-1596-6-102

Figure Lengend Snippet: Survival curves of the expression of combinations of Mucins (n = 44) . Kaplan-Meier curves analyze the relationships between survival and combinations of expression of MUC genes, including the combination of (A) MUC1 +, MUC2 -, MUC5AC +, and MUC6 -; (B) MUC1 +, MUC2 -, MUC5AC -, and MUC6 -; and (C) MUC1 +, MUC2 -, MUC5AC -, and MUC6+ indicated by dashed lines and other combinations indicated by solid lines.

Article Snippet: The solution of 10% goat serum and primary antibody against MUC6 were obtained from Boster Biotech (Wuhan, Hubei, China).

Techniques: Expressing